the study of proteomic salinity responses in barley

responses of plants to salinity stress and development of salt tolerance are extremely complex and various mechanisms appear to be involved. we employed a proteomic approach to study the mechanism of plant responses to salinity in a sensitive cultivar of barley (527 line). three week old seedlings were treated with 300 mm nacl for 3 weeks. total proteins of fourth leaf were extracted and separated by two dimensional gel electrophoresis. 377 protein spots were reproducibly detected including 55 that were up regulated and 69 down regulated. using maldi tof tof ms 25 proteins involved in many cellular functions were identified. these proteins were; oxygen evolving enhancer protein methionine sulfoxide reductase chloroplast rna binding proteins chloroplast localized cyclophilin rubisco ribulose bisphosphate carboxylase/oxygenase activase nascent polypeptide associated complex alpha thioredoxin dehydroascorbate reductase oxalate oxidase like protein or germin like protein profilin universal stress proteins and ribosomal protein.